Neutralization assay of NDV-pseudotyped HIV-Luc virus by the anti-NDV sera.

<p>Sera against NDV were two-fold serially diluted with DMEM. 100 µl titrated NDV-pseudotyped HIV-Luc viruses were mixed with an equal volume of diluted anti-NDV sera. The mixtures were added to 293 T cells in 96-well plates after incubating at room temperature for 1 h. (A) Neutralization of NDV-pseudotyped virus by the immune sera against NDV. Negative serum and cell control refer to the cells infected with a mixture of negative serum from chickens with NDV-pseudotyped virus and cells without pseudovirus infection, respectively; (B) Neutralization efficiency of NDV-pseudotyped HIV-Luc virus by the three anti-NDV sera at an indicated dilution. Results are shown as means ± SD from four independent infections.