Sequence reproducibility by read direction.

<p>A) Histograms of the most common sequence detected in the reverse direction but missing in the forward direction for the MOTIVATE/A4001029 dataset (N = 1521). Less abundant sequences were more likely to be detected only in a single direction compared to sequences with higher abundance. B) Correlation of measured proportion non-R5 for the MOTIVATE/A4001029 dataset between the forward and reverse direction (N = 1521). A linear regression is shown as a solid line. C) Histograms of the most common sequence detected in the forward direction but missing in the reverse direction for the MOTIVATE/A4001029 dataset (N = 1521). D) The lower panel contains a Bland-Altman comparison. Mean difference is plotted as a solid black line and limits of agreement are shown as dotted lines.