Sequence differences by viral load and PCR replication.

<p>A) Percent non-R5 virus detected at varying input copy numbers for a non-homogeneous clinical sample. The dashed line represents the approximate true value for percent of non-R5 virus. B) Measures of viral diversity for 18 and 286 input copies on the non-homogeneous clinical sample amplified with a single PCR and with triplicate PCR. Nucleotide entropies are shown on the left, mean phylogenetic branch length is shown on the right. Estimates tended to be more accurate when the input copy numbers were higher.