MiR-128 directly targets SP1.

<p>(A) Putative binding site of miR-128 within SP1 3′-UTR in human, chimpanzee and mouse, as predicted by TargetScan, PicTar, and MiRDB algorithms. (B) SP1 protein levels in U87 and U251 cells were detected after introduction of miR-128 mimics or scramble oligonucleotides. (C) and (D) In U87 and U251 cells, wild-type groups displayed decreased luciferase activity in comparison with mutant type groups. Data are expressed as the mean ± S.D. *P<0.05.