MiR-127 down-regulates Bcl6 and Setd8 expression.

<p>(<b>A</b> and <b>B</b>) BRL-3A (<b>A</b>) and Huh7 (<b>B</b>) cells transfected with miR-127 mimics or miRNA NC were analyzed by qRT-PCR (left) and Western blotting (right), respectively. Actin was used as a control. (<b>C</b>) Graphic overview of the conserved 3′UTR sequences of Setd8 among rat, mouse and human. The “seed sequences” in the 3′UTRs of Setd8 mRNA were indicated (red box). (<b>D</b>) The repression of Setd8 gene mediated by the 3′-UTR was analyzed by luciferase reporter assay. BRL-3A cells were cotransfected with a luciferase reporter vector containing the 3′-UTR or the mutated 3′-UTR (Mu-UTR) of Setd8 and miR-127 mimics (miR-127) or the negative control (NC). The pGL4.13 control vector was used as a control. The wild-type and mutated miR-127-binding sites in the 3′-UTR of this gene were indicated (left). Data from three independent experiments are shown as the means ± SD. (*<i>P</i><0.05, **<i>P</i><0.01).