IGF1-induced resistance to <i>P. falciparum</i> was reversed by NOS inhibition.

<p>Age- and cohort-matched mosquitoes were fed infected blood meals supplemented with water, L-NAME, 0.13 µM IGF1 or 0.13 µM IGF1+L-NAME as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004231#s4" target="_blank">Materials and Methods</a>. This experiment was replicated four times with separate cohorts of mosquitoes. No significant differences in oocyst intensity were identified among replicate control groups (Kruskal-Wallis, p>0.05), so data were pooled for analysis. Intensity data shown are from infected midguts only. (A) Prevalence of infection (proportion of mosquitoes dissected with at least one <i>P. falciparum</i> oocyst; values indicated above bars). Fisher's exact test was used to compare treatments against L-NAME. (B) Intensity of infection (mean oocysts per infected midgut; values indicated to the right of data for each group). Mann-Whitney test was used to compare treatment groups to matched controls.