<i>Plasmodium</i> oocyst intensity increases in LL3 knockdown mosquitoes.

<p>GFP (control) or LL3 dsRNA was injected into adult female mosquitoes and two days later, surviving mosquitoes were given an infectious blood meal containing <i>P. berghei</i> (A) or <i>P. falciparum</i> (B) parasites. Oocyst numbers were determined respectively at 10 or 7 days following the blood meal. Data from six (<i>P. berghei</i>) or three (<i>P. falciparum</i>) independent experiments were pooled and analyzed by the Mann-Whitney test. The horizontal bars denote the median value for each experimental group. N: number of midguts assayed; Prevalence: percent of midguts that had at least one oocyst. (C) Efficiency of LL3 knockdown and its effect on SRPN6 mRNA abundance were assessed in midguts of <i>P. berghei</i>-infected mosquitoes 24 h PBM. The relative gene expression represents transcript abundance normalized to the GFP control across four separate experiments. Data were compared with the Student's t-test to determine differences in LL3 and SRPN6 expression between GFP control and LL3 knockdown samples. Asterisks denote a <i>P</i> value of <0.05.