The control effect of miRNA21 on bcl-2 after MPT64 treatment RAW264.7.

<p>Macrophages were treated as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100949#pone-0100949-g002" target="_blank">Fig. 2</a>. The miRNA21 level was analyzed by real time-PCR (5A). **<i>P</i><0.01, the PPD group vs the PPD-MPT64 (15 µg/ml) group. The control effect of miRNA21 on bcl-2 was also analyzed by relative luciferase activity in RAW264.7 cells (5B). The wild-type or mutated type of bcl-2-utr was cloned into a pMIR-reportTM luciferase vector. Then, the recombinant plasmids and miR21 were transfected into RAW264.7 cells. The relative luciferase activity was detected. miR-mock and miR335 were used as control miRNAs. *<i>P</i><0.01, the miR21 group vs the miR-mock group after the transfection by wild-type bcl-2-utr. Data shown are representative of three independent experiments.