MiR-23b directly targets the Marcksl-1 3′UTR in macrophages.

<p>Marcksl-1 3′UTR was cloned into a luciferase reporter gene (Luc-Marcksl-1 3′UTR), and transfected into RAW 264.7 cells in the presence or absence of miRNA 23b precursor. The targeting of miRNAs to Marcksl-1 3′UTR was then assessed by measuring luciferase activity in these cells. The miRNA 23b co-transfected with Luc-Marcksl-1 3′UTR significantly reduced luciferase activity in macrophage cells after 24 h of transfection. In contrast, Luc-Marcksl-1 3′UTR had an increase of luciferase activity. Values represent means ± SEM of n = 3 and ***<i>p</i><0.0001.