After surgery, newly innervated NMJs are less abundant, and muscles more atrophic in <i>Celsr3|Emx1</i> than control mice.

<p>A, B: Five months post surgery, left (L, intact side) and right (R, surgery side) biceps brachii were weighted. The R/L wet weight ratio was 97% in control mice versus 80% in mutant mice (**: <i>P</i><0.01, <i>t</i>-test, n = 10 in each group). C–J: Different classes of NMJ in the biceps at 5 months post surgery, stained with anti-NF200 to label regenerating axon (green), and anti-<i>α</i>-BT to label AchR clusters (red). In remodeled NMJs, thin axon terminals innervate some but not all fragmented AchR clusters (arrows in E, I). Neoformed NMJs are characterized by small AchR clusters innervated by thin axons that lack terminal arbors (asterisks in F, J). Quantification in K, 100 NMJs in each group, n = 4 mice; **: <i>P</i><0.01, one-way ANOVA.