No obvious changes in skeletal muscle in merlin-deficient mice.

<p>a, Immunohistochemical stainings of denervated tibialis anterior (TA) muscle sections from wildtype and Nefh-Cre;Nf2^fl/fl mice (n = 3), 5 weeks after crush injury. Laminin staining delineates single muscle fibers. Scale bar 100 μm. b, Quantification of tibialis anterior muscle weight from indicated genotypes (*<i>P</i> < 0.05; n.s. non-significant; n = 3 animals per genotype; mean ± SEM). c, Representative photographs of HE stained tibialis anterior muscle fibers. 5 weeks after sciatic nerve crush injury, ipsilateral (lesion side) and contralateral (control side) muscle tissue was prepared from wildtype (WT) and knockout (Nefh-Cre;Nf2^fl/fl) animals. Scale bar 100 μm. d, Quantification of the minimal fiber feret of tibialis anterior muscles from indicated genotypes was analyzed (*<i>P</i> < 0.05; **<i>P</i> < 0.01; n.s. non-significant; n = 4 animals per genotype; mean ± SEM). e, Representative images of neuromuscular junctions from extensor digitorum longus (EDL) muscle stained with Bungarotoxin (binds to nicotinic acetylcholine receptor) and neurofilaments and SV2. Scale bar 20 μm. f, Quantification of fully occupied endplates in extensor digitorum longus (EDL) muscles from indicated genotypes (n.s. non-significant; n = 3 animals per genotype; mean ± SEM).