MCK-UCP1 mice show delayed functional recovery after nerve crush.

<p>A–B: Sciatic functional index (A) and grip strength (B) in 1-month-old wild-type (empty diamonds) or MCK-UCP1 mice (filled diamonds) after sciatic nerve crush, performed at day 0. Grip strength was expressed as the percentage of ipsilateral strength relative to the contralateral limb. *, p<0.05 vs corresponding wild type; #, p<0.05 vs day 0 (n = 12 mice per group). C: Mass of contralateral (empty columns) and ipsilateral (black columns) gastrocnemius from wild-type (Wt) or MCK-UCP1 mice 30 days after sciatic nerve crush. *, p<0.05 vs contralateral muscle (n = 5 mice per group). D: quantification of the percentage of synaptophysin positive NMJs as shown in E, *, p<0.05 vs wild-type. (n = 5 mice per group). E: representative photomicrographs showing AchR (red) and neurofilament/synaptophysin (green) staining in contralateral and ipsilateral gastrocnemius from wild-type (Wt) or MCK-UCP1 mice 8 days after sciatic nerve crush (e) and quantification of the experiment (d). Note the denervated endplates (stars) and the retracted nerve fibers (arrowheads). Scale bar, 20 µm.