<p>Left: TCRγδ+ T cells; Right: CD3− cells. Non-celiac patterns: A, Normal pattern; and B, Decrease of CD3− cells. Celiac patterns: C, Increase of TCRγδ+ T cells <i>plus</i> decrease of CD3− cells; and D, Increase of TCRγδ+ T cells only.</p
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
Shown are gating strategies for the analysis of (A), human immune cell subsets (hCD45+CD3+: T cells,...
<p>A. Flow cytometry dot plots represent lymphocytes region (P1), frequency of CD4<sup>+</sup>CD25<s...
Representative plots showing non-circulating T follicular helper cells (cTfh) populations as CD3+CD4...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>T-cell subsets were based on the CD27 and CD45RO cell surface markers. A HAT patient before and a...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>Fresh biopsies were digested to release cells and subsequently stained with antibodies to determi...
<p>Representative flow cytometry plots of T-cell activation- and exhaustion markers in HIV-HCV coinf...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
Representative plots showing activated B cells (ABC) as CD19+CD10-IgD-CD71+CD38-CD20+ and antibody s...
Gating strategy for T and B cell panels are shown. Naïve CD4+ or CD8+ T cells were defined as CD28+/...
<p>Based on the gating and defined subpopulations illustrated in <a href="http://www.plosone.org/art...
<p>Representative analyses from a patient with Tb (A) and an LTBI (B) are shown. Open red circles an...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
Shown are gating strategies for the analysis of (A), human immune cell subsets (hCD45+CD3+: T cells,...
<p>A. Flow cytometry dot plots represent lymphocytes region (P1), frequency of CD4<sup>+</sup>CD25<s...
Representative plots showing non-circulating T follicular helper cells (cTfh) populations as CD3+CD4...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>T-cell subsets were based on the CD27 and CD45RO cell surface markers. A HAT patient before and a...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
<p>(A) Profiles of representative TIL cultures (specimen M27, TIL cultures 2 and 8) stained for CD3,...
<p>Fresh biopsies were digested to release cells and subsequently stained with antibodies to determi...
<p>Representative flow cytometry plots of T-cell activation- and exhaustion markers in HIV-HCV coinf...
<p>The numbers represent percentage of cells within the gates. At least 10<sup>6</sup> total cells w...
Representative plots showing activated B cells (ABC) as CD19+CD10-IgD-CD71+CD38-CD20+ and antibody s...
Gating strategy for T and B cell panels are shown. Naïve CD4+ or CD8+ T cells were defined as CD28+/...
<p>Based on the gating and defined subpopulations illustrated in <a href="http://www.plosone.org/art...
<p>Representative analyses from a patient with Tb (A) and an LTBI (B) are shown. Open red circles an...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
Shown are gating strategies for the analysis of (A), human immune cell subsets (hCD45+CD3+: T cells,...
<p>A. Flow cytometry dot plots represent lymphocytes region (P1), frequency of CD4<sup>+</sup>CD25<s...