Representative flow cytometry plots.

<p>Representative flow cytometry plots of T-cell activation- and exhaustion markers in HIV-HCV coinfected patients, HCV mono- and HIV mono-infected patients and healthy controls. A: gating of CD4⁺ and CD8⁺ T-cells by lymphocyte-gate (left panel), CD3-gate (middle panel) and gates for CD4⁺ or CD8⁺ T-cells (right panel). B-E: representative flow cytometry plots of a healthy control (left), HIV-HCV coinfected (middle left), HCV monoinfected (middle right) and HIV -monoinfected patient (right) showing (B) activated CD8⁺ T-cells; (C) Fas-positive CD4⁺ T-cells; (D) PD-1 positive CD4⁺ T-cells and (E) Tim-3 positive CD8⁺ T cells. Percentages are depicted in the right upper corner.