The route of IMI-Gel application influences vaccination efficacy.

<p>C57BL/6 mice were shaved on their backs and received the following treatments: untreated (untreated control), IMI-Gel with SIINFEKL (100 µg) (IMI-Gel TCI) on two consecutive days, IMI-Gel alone (IMI-Gel without SIIN) on two consecutive days, oral IMI-Gel (50 mg) with SIINFEKL (100 µg) on two consecutive days (IMI-Gel p. o.) or s. c. once with IMI-Gel (100 mg) diluted with SIINFEKL (200 µg) and distilled water into the neck. <b>A)</b> The frequency of peptide-specific CD8⁺ T cells in the blood (mean and SD) and <b>B)</b><i>in vivo</i> cytolytic activity 24 hours (mean and SD) after transfer of peptide-loaded target cells was assessed. Depicted are the cumulative results of two independent experiments with n = 7 for IMI-Gel treated groups and n = 4 for controls. *Significant difference with p<0.05 by Students <i>t</i> test.