Evaluation of specific cytotoxicity induced by vaccination with MIS416/SIINFEKL conjugates.

<p>Six mice were used in each group and they were vaccinated with Conjugation A or B (100 μg), MIS416 (100 μg) plus SIINFEKL (2 μg), MIS416 alone (100 μg), PBS (100 μL), CpG plus SIINFEKL (2 μg) or CpG plus Ovalbumin (10 μL of OVA + 50μg of CpG). After 1 week mice were challenged with two population of splenocytes (1 x 10⁷ cells, pulsed with SIINFEKL or not) and after two days specific cytotoxicity was evaluated. CpG + OVA was used as positive control while PBS and MIS416 were negative controls. Specific cytotoxicity as a percentage was calculated using the ratio between cells stained with VPD450 (pulsed with SIINFEKL) and with CFSE (unpulsed), of the vaccinated groups compared to the control group (PBS).