Bacterial burden is significantly reduced at later time points.

<p><b>A)</b> Quantitative measurement of leptospiral DNA (16S) in kidney samples from WT (WT+LIC) or Daf1^−/− (DAFKO+LIC) infected animals with 10⁶ bacteria at 14 and 90 dpi. Bars represent standard error mean (SEM) of assays from a group of five to seven mice. Three pieces of each organ were analyzed in triplicate q-PCR and normalized to host cell number; **<i>p</i><0.01, <b>^ns</b><i>p</i>>0.05. <b>B–C)</b> Immunohistochemistry with antiserum specific for LipL32 (x200) of kidney sections of uninfected (WT+PBS and DAFKO+PBS) and infected WT (WT+LIC) and Daf1^−/− (DAFKO+LIC) animals at 14 and 90 dpi. Arrows indicate representative positive foci.