Renal bacterial burden at the beginning of the leptospiruric phase and immunohistochemistry analysis.

<p><b>(A)</b> C57BL/6J male weanlings received 0.25 mg of non-immune rat IgG or purified anti-Ly6G rat mAb1A8 every 48 h and 200 μL of 1x10⁷/mL pathogenic <i>Leptospira interrogans</i> serovar Copenhageni (LIC). Kidney samples of non-depleted and neutrophil-depleted LIC-infected mice were collected at 14 days post-infection. Leptospiral DNA was quantified by real-time PCR and normalized to host cell number. Bars represent mean ± SEM of two independent experiments; **<i>p</i> <0.01. <b>(B)</b> Positive renal tubules were counted under a 40x field. Bars represent mean ± SEM of three independent observations; **<i>p</i> <0.01. <b>(C)</b> Representative positive slides of immunohistochemical staining of LipL32 in kidney from LIC-infected mice rat IgG-treated or mAb1A8-treated. The results are representative of two different experiments (n = 6–10 mice). Slides were counterstained with hematoxylin. Scale bar indicates 50 μm.