Analysis of derivative <i>ecpA</i> mutants.

<p>(A) Immunogold labeling of ECP produced by (a) CFT073, (b) CFT073 Δ<i>ecpA</i>, (c) CFT073 Δ<i>ecpA</i> (pMAT9), (d) F11, (e) F11 Δ<i>ecpA</i>, and (f) F11 Δ<i>ecpA</i> (pMAT9) with anti-ECP antibodies and rabbit anti-IgG gold conjugate. Scale bars represent 500 nm. (B) Immunofluorescence of UPEC strains with anti-ECP antibodies and rabbit anti-IgG and Alexa-Fluor 488-conjugated secondary antibody (green). Cellular and bacterial DNA was stained with propidium iodide (red). Images were taken with a 100× objective and magnified 5X. (C) Immunoblot showing EcpA production in CFT073, CFT073 Δ<i>ecpA</i> (pMAT9), F11 and F11 Δ<i>ecpA</i> (pMAT9), but not in the CFT073 Δ<i>ecpA</i> or F11 Δ<i>ecpA</i> mutants. DnaK detection was used as loading control of equal amounts of bacteria onto the gels. (D) Motility of UPEC strains in motility agar. Except for the CFT073 Δ<i>fliC</i> all other strains showed motility.