Detection of ECP synthesis and expression on APEC χ7503 strain and its derivatives.

<p>ECP expression on the surface of bacteria by immunoelectron microscopy (<b>A</b>) and ECP synthesis in total bacterial Western blotting using anti-ECP antibodies (<b>B</b>). The ECP synthesis and expression is shown in the wild-type strain. The single and double mutants were deficient in ECP synthesis and expression. The ECP synthesis and expression were restored in the complemented strains. (Scale bars, 500 nm.). Detection of DnaK with anti-DnaK antibody was used to ensure equal amounts of antigen tested.