Summary of the EMSA and MST results.

<p>The respective MST binding curves are plotted on the left, the fluorescence channel depicting the EMSA of R-loop substrate is shown in the middle panel and the merge of the EMSA fluorescence channels revealing the substrate and the controls is shown on the right. The concentration of S9.6 antibody used for the individual experiments is shown on top of the gel. The nucleic acid-antibody complexes are indicated by a triangle and control R-loops (ctrl1 and ctrl2) are indicated on the right side of the gel. <b>(A)</b> Analysis of the R-loop 23GC52. The double stranded DNA D23GC17L served as negative control (ctrl1, red). <b>(B)</b> Analysis of the R-loop 15GC0U. The R-loop 23GC52L served as positive control (ctrl, red). <b>(C)</b> Analysis of the R-loop 15GC0A. The double stranded DNA D23GC17L (ctrl1, red) served as negative control and the R-loop 23GC52L (ctrl2, blue) served as positive control. <b>(D)</b> Analysis of the R-loop 29GC21. The R-loop 15GC0A (ctrl, red) served as positive control. <b>(E)</b> Analysis of the R-loop 16GC80. The double stranded DNA D23GC17L (ctrl, red) served as negative control. <b>(F)</b> Analysis of the R-loop 22GC90. The double stranded DNA D23GC17L (ctrl, red) served as negative control. <b>(G)</b> Analysis of the R-loop 22GC10. The double stranded DNA D23GC17L (ctrl, red) served as negative control.