EMSA shows the DNA binding properties of <i>CgTR in vitro</i>.

<p>(A) Recombinant CgTR (rCgTR) bound to probes DR0-DR5. Lanes 1, 5, 9, 14, 18, and 22 contain the reaction mixture without rCgTR as negative controls; Lanes 2, 6, 10, 15, 19, and 23 contain rCgTR; Lanes 3, 7, 11, 16, 20, and 24 contain rCgTR with a 100 fold concentration of cold probe; Lanes 4, 8, 12, 17, 21, and 25 contain rCgTR with antibody. Lane 13 contains rCgTR as an internal reference in the second plate to compare the binding intensity of DR0-DR5. As only one band was discerned, it is difficult to determine whether the bands are a homodimer or monomer. (B) EMSA demonstrated that CgTR can bind to the CgDR5. Lane 1 contains the reaction mixture without rCgTR as a negative control; Lane 2 contain rCgTR; Lane 3 contains rCgTR with a 100 fold concentration of cold probe; Lane 4 contains rCgTR with antibody. (C) Recombinant CgTR containing amino acids from 1 to 240 (rCgTR1-240) can bind to DR2 as a homodimer (ho) and monomer (mo). Lane 1 contains the reaction mixture without rCgTR1-240 as a negative control; Lane 2 contains rCgTR1-240; Lane 3 contains rCgTR1-240 with a 100 fold concentration of cold probe; Lane 4 contains rCgTR1-240 with antibody.