Effect of B[<i>α</i>]P on LSR, LDL-R and ABCA1 protein and mRNA levels in Hepa1-6 cells.

<p><b>A)</b> Hepa1-6 cells were incubated at 37°C for up to 2 h in the absence or presence of the indicated concentrations of B[<i>α</i>]P, after which cell lysates and were prepared as described in Methods. Representative immunoblots (for LSR and ABCA1, results of 1-h incubation are shown; for LDL-R, results for 2-h incubation are shown) and corresponding densitometric analyses using β-tubulin as internal control are shown (one-way ANOVA, *<i>P</i><0.05, **<i>P</i><0.01, as compared to 0 µM B[<i>α</i>]P, n = 3 different cell preparations using duplicate or triplicate wells). <b>B)</b> mRNA levels of LSR, LDLR and ABCA1 were determined in cells incubated with the indicated concentrations of B[<i>α</i>]P using real-time PCR, as described in Materials and Methods. Results are shown (n = 4 in triplicate) of LSR, LDLR and ABCA1 mRNA expression relative to HPRT, used as reference housekeeping gene. It should be noted that there was no significant changes in HPRT expression under the different conditions (**<i>P</i><0.01 as compared to 0 µM B[<i>α</i>]P).