Effect of B[<i>α</i>]P on mouse hepatic LDL-R, LSR, and ABCA1 protein and mRNA levels.

<p><b>A)</b> Immunoblots to detect LDL-R, LSR, and ABCA1 were performed on protein solubilized from liver total membranes isolated from control and B[<i>α</i>]P-treated mice. Blots are shown with corresponding densitometric analysis (Student's t-test, *<i>P</i><0.05, **<i>P</i><0.01 B[<i>α</i>]P as compared to control groups, n = 7 for each group). <b>B)</b> mRNA levels of liver LSR, LDLR and ABCA1 isolated from control and B[<i>α</i>]P-treated mice were determined using real-time PCR, as described in Materials and Methods. Results are shown for LSR, LDLR and ABCA1 mRNA expression relative to HPRT, used as reference housekeeping gene. There was no significant changes in HPRT expression under the different conditions (**<i>P</i><0.01 as compared to control mice; triplicate determinations of n = 4 per group).