Theoretical models to fit experimental autocorrelation data of PTPD1-GFP obtained by FCS.

<p>The upper panel shows the experimental autocorrelation function (<i>G</i>(<i>τ</i>)) (blue line), and fits of data to the theoretical three-dimensional (3D) or anomalous two-dimensional (2Da) models (red and green lines, respectively). <i>τ</i> indicates the delay time in seconds. The lower panel shows residuals (<i>R</i>) of each fit (red and green lines). Fits of data indicate an anomalous two-dimensional Diffusion of PTPD1. This model is used to obtain the translational diffusion (τ_d) and to calculate the diffusion coefficient as explained in <i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103203#pone.0103203.s005" target="_blank">Methods S1</a></i>. The inset shows the cell analysed, in which a red cross points the position of the confocal volume. Calibration bar of the fluorescence intensity is shown.