Diffusional mobility of Htt fragments in Sn56 cells.

<p>In A, the autocorrelation curves for cytosolic GFP, HttQP25, HttQP47, and HttQP103 were measured 72h after transfection. In cells expressing HttQP47 and HttQP103, the diffusional mobility was measured in cells without inclusions. Curves were normalized using the Confocor2 program. In B, the residuals for each of the autocorrelation curves shown in A. In C, diffusion coefficients of GFP, HttQP25, HttQP47, and HttQP103 were measured 72h after transfection. The diffusion coefficients and standard deviations were calculated by averaging a minimum of 4 data sets each obtained from a different transfection with each data set consisting of a minimum of 10 cells. **, <i>p</i><0.005 determined by Student's <i>t</i>-test of significant difference between GFP and HttQPs diffusion coefficient.