Multispot FCS analysis.

<p>For each sample, 8 donor autocorrelation functions (ACFs) and for samples with sufficient acceptor signal (7d, 12d and 17d), 8 acceptor ACFs, were fitted with a 2D diffusion model with multi-exponential afterpulsing components. Additionally, for all samples, the cross-correlation function (CCF) of the 8 donor and acceptor signals were computed. Each curve was fitted with the respective model described in the text. (A) Diffusion times for the donor ACFs. (B) Diffusion times for the acceptor ACFs. (C) Diffusion times for the donor-acceptor CCFs. A significantly shorter diffusion time is observed for the 22d sample in both the ACFs and CCFs. Sample 7d is characterized by a significantly larger diffusion time in the CCF only.