MiR-31 is overexpressed in cutaneous squamous cell carcinoma.

<p>(A) qPCR analysis of miR-31 in healthy skin, actinic keratosis lesion and cSCC in fresh-frozen clinical material. ***p<0.001, Mann-Whitney U-test. (B) qPCR analysis of miR-31 levels in archived, formalin-fixed paraffin-embedded healthy skin and cSCC. ***p<0.001, Mann-Whitney U-test. (C) In situ hybridization (ISH) was performed on a normal skin and skin SCC sections using DIG-labeled locked nucleic acid (LNA)-based probes for miR-31. Scrambled LNA-probe was used as control. Representative micrographs for miR-31 expression in healthy skin (Healthy) and cSCC tissues (SCC) are shown. Scale bar: 50 µm.