Relative changes in expression of selected mRNAs and mature miRNAs in healthy skin and SCCs.

<p>For each group, five samples were analyzed by qRT-PCR, representing ten individuals. Experiments were performed in quadruplicates. Data are based on C_T values normalized to ACTB and RNU6B for mRNAs and miRNAs, respectively. (A) Fold differences in expression relative to average of expression in healthy skin samples ± S.D. are indicated for each assay on a logarithmic scale. The Mann-Whitney <i>U</i> test was used to calculate P values (two-tailed; the single asterisk denotes a P value<0.05). (B) Based on the normalized expression levels, Spearman rank correlation coefficients (r) between relative expression levels of VEGFA and each of the indicated miRNAs were calculated over all samples. P values (one-tailed) are indicated.