The levels of miR-31 expression in CRC cells and tissues by qRT-PCR or <i>in</i><i>situ</i> hybridization and its prognostic value in patients with CRC.

<p>(A) Expression levels of mature miR-31 in paired CRC and adjacent normal tissues. (B) The miR-31 expression in CRC tissues with or without metastases relative to match-normal tissues. nmCRC denotes CRC tissues without metastases; mCRC denotes CRC tissues with lymph node metastases. (C) Expression of miR-31 in CRC cell lines and subclones. miRNA abundance was normalized to U6 RNA. (D) Expression analysis of miR-31 in normal colorectal mucosa and CRC tissues by <i>in </i><i>situ</i> hybridization. (a) Negative expression of miR-31 in normal colorectal mucosa; (b) Low expression of miR-31 in CRC tissues; (c, d) High expression of miR-31 in CRC tissues; (e) negative control; (f) Kaplan-Meier analysis for survival of patients with CRC according to miR-31 expression. Log Rank = 6.682, <i>p</i> = 0.010.