CHIKV suppresses <b>NF-κB</b> activity through miR-146a.

<p>Increase in miR-146a expression levels suppresses the activation of NF-κB. 2 µg of NF-κB -FLuc plasmid was co-transfected with 700 ng of pCMV-β-gal plasmid in HEK 293 T cells for reporter assay. 6–8 hours post transfection, cells were infected with CHIKV (MOI 2) and harvested 32 hours post infection for Luciferase assay. (<b>A</b>) The graph bars are representing relative luciferase activity of NF-κB in control and CHIKV infected primary human synovial fibroblasts after normalization with β-galactosidase expression units showing decreased NF-κB activity. (<b>B</b>) The graph bars representing relative luciferase units in miR-146a overexpression. Transfection of miR-146a decreases NF-κB activity compared to controls and scramble miR-146a. (<b>C</b>) HEK cells were infected with CHIKV after the 24 hours of anti-miR-146a transfection. Anti-miR-146a transfection rescues the activity of NF-κB even in cells infected with CHIKV. The graph bars are showing elevated luciferase activity in anti-miR-146a and anti-miR-146a+CHIKV infection. All experiments were performed in biological triplicates and data represented as mean ± SEM. (*for p value≤0.05, **for p value≤0.005).