MiR-196a directly targets NFKBIA gene.

<p>(A) NFKBIA is a potential target gene of miR-196a predicted by computational analysis. (B) Representative western blot analysis showed the relationship between miR-196a expression and endogenous NFKBIA protein level. The inhibition of miR-196a in PANC-1 cells increased endogenous NFKBIA protein level, whereas overexpression of miR-196a in BxPC-3 cells attenuated endogenous NFKBIA protein level. (C) Insertion of NFKBIA3′UTR target sequences in a luciferase reporter vector leaded to diminished luciferase activity in presence of miR-196a in PANC-1 cells 24 h after co-transfection. Histograms showed the values resulting as the average ± SD from three independent co-transfections (*, <i>P</i><0.05).