WGCNA analysis of heart RNAseq data.

<p>(A–D) Hierarchical cluster trees of the average linkage in the dissimilarity topological overlap matrices. Each vertical line correlates to a gene. The height is a measure for the dissimilarity based on the topological overlap. The first band under the dendrogram is a visual representation of the identified modules. The second band indicates the correlation with genotype based on the gene significance for each gene. Red is positively correlated with genotype, blue is negatively correlated. (A) Hierarchical cluster tree for WT versus R6/2 at 4 weeks of age. (B) Hierarchical cluster tree for WT versus R6/2 at 15 weeks of age. (C) Hierarchical cluster tree for WT versus <i>Hdh</i>Q150 at 8 months of age. (D) Hierarchical cluster tree for WT versus <i>Hdh</i>Q150 at 22 months of age. (E–H) Modules that were highly correlated with genotype in the respective networks are shown and are ranked according to their correlation with genotype. Size is the number of genes for each module. <i>P</i>adj gives the Benjamini Hochberg corrected significance value for each module. (E) Modules in the R6/2 network at 4 weeks of age. (F) Modules in the R6/2 network at 15 weeks of age. (G) Modules in the <i>Hdh</i>Q150 network at 8 months of age. (H) Modules in the <i>Hdh</i>Q150 network at 22 months of age. (I–K) Preservation analysis of late stage R6/2 and <i>Hdh</i>Q150 networks. The Z-summary is a measure for module preservation. Values less than 2 (red lines) indicate no preservation, between 2 and 10 (blue lines), module structures are preserved and above 10 the module structure is highly preserved. (I) Preservation of R6/2 modules at 15 weeks in the <i>Hdh</i>Q150 network at 22 months. (J) Preservation of <i>Hdh</i>Q150 modules at 22 months in the R6/2 network at 15 weeks. (K) Preservation of R6/2 modules at 15 weeks in a R6/2 network of skeletal muscle at 12 weeks.