Weighted gene correlation network analysis (WGCNA) identifies lncRNAs-mRNA co-expression network modules.

(A) The dendrogram shows 19 lncRNAs-mRNA co-expression modules identified using WGCNA. (B) The heatmap illustrates the correlation between the identified modules and various biological traits. Each column represents a trait, while each row corresponds to a module eigengene. The strength and direction of the association between each trait and eigengene are indicated by a Pearson’s correlation coefficient, with the corresponding P-value provided in parentheses. The color gradient within each cell reflects the correlation, with blue signifying a strong negative correlation and red indicating a strong positive correlation. (C) Heatmap of lncRNAs in the turquoise and yellow modules during 14–26 dpi in male and female worms. Red and blue represent high and low gene expression levels, respectively. (D) Gene ontology (GO)) enrichment of the coding genes in turquoise and yellow modules. Color intensity represents the magnitude of -log10(adjusted P-value) (Fisher’s exact test). Only GO terms with adjusted P-value −4 are shown.