Measurement of microparticle TF procoagulant activity and FVIIa-AT levels in plasma following LPS administration in wild-type mice.

<p>Wild-type mice (C57BL/6J) were injected with saline or LPS (5 mg/kg body weight) intraperitoneally. 5 h after saline or LPS administration, blood was obtained from these mice by cardiac puncture, and plasma samples were processed for isolation of microparticles to measure TF procoagulant activity (A) or for determination of FVIIa-AT levels (B). Procoagulant activity measured in factor Xa generation assay in the absence or presence of mouse TF mAb (10 µg/ml, preincubated for 30 min) (n = 5). *Indicates the values differ in statistically significant manner between the control and LPS-treated mice. The concentration of FVIIa-AT (ng/ml) reflects ng of FVIIa complexed with AT. *, <i>P</i><0.05; <i>***, P</i><0.001; ns, not statistically significant.