Validation of microarray data by pyrosequencing.

<p>Validation of significant methylation differences identified by microarray (450 K) by pyrosequencing (PS), showing the average methylation level of CpG sites within the following genes (probe ID, genic location): (a) LY86 (cg02212836, first exon), (b) HIPK3 (cg25600606, gene body), and (c) LCN2 (cg14615559, TSS200). * = FDR<0.05, 450 K; * = p<0.05, PS, Wilcoxon rank-sum test. Error bars represent the standard error of the mean.