<p>Pxa2_NBD was fused to Gal4 AD and Pxa2_CT was fused to Lex BD in yeast two-hybrid plasmids. Two-hybrid analysis was performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104892#s4" target="_blank">Materials and Methods</a>.</p
Two-hybrid screening is a standard method used to iden-tify and characterize protein-protein interac...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yield...
<p>(A) Two-hybrid analysis of the interaction between the CT of Pxa2_NBD-CT (Pxa2_CT) and Pxa1_NBD. ...
<p>The EGY48 (pSH18-34) reporter strains that express the indicated hybrid proteins (with the Pxa2 f...
<p>(A) Identification of recombinant plasmid pAS2-1/FHL3d4 by restriction analysis. M:DNA marker; La...
<p>(A) An illustrative yeast two hybrid Interaction assay involving wild type PipX, P<sub>II</sub>, ...
<p>Upper panels: yeast THY-AP4 strain was transformed with plasmids pBTM-R6 wt (LexA-R6), pBTM-R6 RA...
<p>The pGBKT7-TaCBLs and pGADT7-TaCIPK2 plasmids were co-transformed into the AH109 yeast strain and...
<p>(A) Multiple sequence alignment of the C-terminal amino acid sequences of the peroxisomal ABC tra...
The yeast two-hybrid system was originally designed to detect protein–protein interactions using yea...
The yeast two-hybrid system was originally designed to detect protein–protein interactions using yea...
The yeast two-hybrid system was originally designed to detect protein–protein interactions using yea...
<p>(<b>A, B</b>) Summary of Y2H assay results. Y2H Gold yeast strains co-transformed with the indica...
<p>AH109 yeast cells containing plasmids AD+BD, AD-PyMYB10+BD, AD-PyMYB10.1+BD, AD+BD-PybHLH, AD-PyM...
Two-hybrid screening is a standard method used to iden-tify and characterize protein-protein interac...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yield...
<p>(A) Two-hybrid analysis of the interaction between the CT of Pxa2_NBD-CT (Pxa2_CT) and Pxa1_NBD. ...
<p>The EGY48 (pSH18-34) reporter strains that express the indicated hybrid proteins (with the Pxa2 f...
<p>(A) Identification of recombinant plasmid pAS2-1/FHL3d4 by restriction analysis. M:DNA marker; La...
<p>(A) An illustrative yeast two hybrid Interaction assay involving wild type PipX, P<sub>II</sub>, ...
<p>Upper panels: yeast THY-AP4 strain was transformed with plasmids pBTM-R6 wt (LexA-R6), pBTM-R6 RA...
<p>The pGBKT7-TaCBLs and pGADT7-TaCIPK2 plasmids were co-transformed into the AH109 yeast strain and...
<p>(A) Multiple sequence alignment of the C-terminal amino acid sequences of the peroxisomal ABC tra...
The yeast two-hybrid system was originally designed to detect protein–protein interactions using yea...
The yeast two-hybrid system was originally designed to detect protein–protein interactions using yea...
The yeast two-hybrid system was originally designed to detect protein–protein interactions using yea...
<p>(<b>A, B</b>) Summary of Y2H assay results. Y2H Gold yeast strains co-transformed with the indica...
<p>AH109 yeast cells containing plasmids AD+BD, AD-PyMYB10+BD, AD-PyMYB10.1+BD, AD+BD-PybHLH, AD-PyM...
Two-hybrid screening is a standard method used to iden-tify and characterize protein-protein interac...
<p>(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast t...
<p>(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yield...