ECTV and ECTV-Δ005 inhibit production of NF-κB regulated transcripts.

<p>HeLa cells were mock-infected or infected with ECTV or ECTV-Δ005 at a MOI of 5. At 12 hours post infection cells were stimulated with TNF hours post infection cells were stimulated with TNFα. RNA samples were collected at the indicated time points post-TNFα treatment. Samples were reverse transcribed, followed by real time PCR analysis for relative levels of (A) TNFα, (B) IL-1β, and (C) IL-6 transcripts, as compared to GAPDH. Time courses were performed in triplicate and plotted as the average with standard error.