ECTV encoded EVM005 inhibits p65 nuclear accumulation.

<p>(A and B) HeLa cells were mock-transfected or transfected with pcDNA-Flag-EVM005 or pcDNA-Flag-EVM005(1-593). At 12 hours post transfection cells were treated with 10 ng hours post transfection cells were treated with 10 ng ng/ml TNFα (A) or 10 ng ng/ml IL-1β (B) for 20 minutes. Cells were fixed and stained with anti minutes. Cells were fixed and stained with anti-Flag to detect EVM005 and anti-p65. Cells were visualized by immunofluorescence. (C) Data was quantified by counting more than 50 cells in triplicate.