Directed Comparison of abundant miRNAs.

<p>All miRNAs with at least one data point had a CT≤28 were selected, normalized to stable small RNAs, and the median CT across the 2, 4, and 8 hr time point was calculated. These were further standardized to yield a Z score, which reflects relative changes. The Z score is shown on the vertical axis and miRNAs on the horizontal axis. The Z scores were ordered by the level of uninfected HEK293-Null (A, B) or HEK293-TLR3 (C, D) cells. Panels (A) and (C) compare the response of WNV to formalin-inactivated virus. Panels (B) and (D) compare the response of WNV infection to polyI:C stimulation. (E) Caliper LabChip analysis of a highly expressed and undetected miRNA. qPCR products of miRNA assays were run on a Caliper LabChip GX and the resulting electropheregram is shown. The sample yields a single peak corresponding to the expressed miRNA and two assay marker bands (UM – upper marker and LM – lower marker). The undetected miRNA electropheregram shows only an upper and lower marker peak with very little to no background signal, confirming the high specificity of this assay.