PARG regulates transcriptional responses to TGFβ.

<p>(<b>a–c</b>) Real-time RT-PCR analysis of endogenous <i>fibronectin (FN1)</i> (a), <i>PAI-1</i> (b) and control <i>PARP-1</i> (c) mRNAs in HaCaT cells transiently transfected with the indicated siRNAs (bottom of panel c) prior to stimulation (or not) with 5 ng/ml TGFβ1 for 9 h. The data are graphed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103651#pone-0103651-g007" target="_blank">Fig. 7g, h</a>. (<b>d–f</b>) Real-time RT-PCR analysis of endogenous <i>fibronectin (FN1)</i> (d), <i>PAI-1</i> (e) and control <i>PARG</i> (f) mRNAs in HaCaT cells transiently transfected with the indicated siRNAs (bottom of panel f) prior to stimulation (or not) with 5 ng/ml TGFβ1 for 9 h. The data are graphed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103651#pone-0103651-g007" target="_blank">Fig. 7g, h</a>. Stars (panels b–g) indicate statistical significance, <i>p</i><0.05. The figure shows representative experiments from four or more repeats.