Influence of ParB on gene expression in the <i>parS1-4</i> region.

<p>(<b>A</b>) Mean level of expression of <i>dnaA</i>-<i>def</i> (<i>PA0001</i>-<i>PA0019</i>) genes in ParB+ and ParB+++ cells relative to EV as revealed by microarray analysis. Filled markers indicate statistically different expression relative to EV (<i>p</i>-value < 0.05 in ANOVA test). Arrangement of the genes in the chromosome is shown below. Operons (according to the DOOR 2.0 database [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181726#pone.0181726.ref073" target="_blank">73</a>]) are marked in grey. (<b>B</b>) Expression of <i>PA0001</i>-<i>PA0016</i> <b>(</b><i>dnaA</i>–<i>trkA</i>) genes in ParB+ and ParB+++ cells relative to EV cells. (<b>C</b>) RT-qPCR analysis of expression of <i>dnaA</i>–<i>trkA</i> genes in <i>parB</i>_null and <i>parS</i>_null strains relative to WT cells. Cells were grown in L broth. (<b>D</b>) RT-qPCR analysis of expression of <i>dnaA</i>–<i>trkA</i> genes in <i>parS</i>_null (pKGB9 <i>araBADp</i>-<i>parB</i>) and <i>parS</i>_null (pKGB8 <i>araBAD</i>p) relative to EV [PAO1161 (pKGB8 <i>araBAD</i>p)]. Cells were grown in L broth supplemented with chloramphenicol and 0.02% arabinose. RT-qPCR data represent mean ±SD from three biological replicates. Filled symbols indicate significantly different expression (<i>p</i>-value <0.05 in two-sided Student’s <i>t</i>-test assuming equal variance) relative to the control cells labelled as blue squares. The differences in expression of genes in <i>parS</i>_null (pKGB9) strain relative to <i>parS</i>_null (pKGB8) strain are not statistically significant.