Influence of ParB level on expression of genes adjacent to <i>parS6</i>.

<p>(<b>A</b>) Mean level of expression of <i>PA0488</i>–<i>PA0498</i> genes in ParB+ and ParB+++ cells relative to EV cells (microarray data). Filled markers indicate statistically different expression relative to EV (<i>p</i>-value < 0.05 in ANOVA test). Operons (according to the DOOR 2.0 database [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0181726#pone.0181726.ref073" target="_blank">73</a>]) are labelled with different shades of grey. (<b>B</b>) RT-qPCR analysis of expression of <i>PA0492</i>, <i>PA0493</i> and <i>PA0494</i> genes in <i>parB</i>_null and <i>parS</i>_null strains relative to WT cells. Data represent mean ±SD from three biological replicates. The differences between strains / conditions are not statistically significant (<i>p</i>-value > 0.05 in two-sided Student’s <i>t</i>-test assuming equal variance).