PLA of endogenous PARP-1 and PARP-2 ADP-ribosylation after TGFβ stimulation in HaCaT cells.

<p>(<b>a, c</b>) HaCaT cells were analyzed with PLA using antibodies against PARP-1 and PAR chains (<b>a</b>) or antibodies against PARP-2 and PAR (<b>c</b>) after stimulation with vehicle (0 min) or with 2 ng/ml TGFβ1 for the indicated time periods. Specific RCA signals were detected in the nuclei. PLA with single antibodies against PARP-1 or PAR are shown as controls. PLA images are shown as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103651#pone-0103651-g001" target="_blank">Fig. 1a</a>. (<b>b, d</b>) Quantification of the experiments shown in panels (a, c) following the histogram method of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103651#pone-0103651-g001" target="_blank">Fig. 1b</a>. The figure shows a representative experiment from three or more repeats.