<p>Spectra from 250 to 800 nm of 10 µM proteins measured by UV-visible spectrometry and inset shows CBB staining of purified proteins (400 ng) after SDS–PAGE (12% gel) with molecular mass markers given in kDa on left.</p
<p>Spectra of RAL 20 µM (black) together with 20 µM LTR32 (green), 20 µM LTR34 (blue), 20 µM LTR30 (...
<p>Rtms5<sup>Y67F</sup> (A) and Rtms5<sup>Y67F/H146S</sup> (B) were diluted to a final protein conce...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
<p>Absorption spectra of purified proteins were taken between 260–600 nm wavelengths. A; VcCry1-MBP....
<p>Spectra were normalized to the 280 nm absorption for each protein. <b>A.</b> Absorption spectra o...
<p>A) Protein purity assessed on SDS-PAGE. MW: molecular weight standard from Euromedex; 1: Cfp21 WT...
<p>(A, B) Far-UV CD spectra and fluorescence spectra of folded and unfolded NS1 (full and dashed lin...
<p>(A) SDS-PAGE of each purified recombinant protein. (B) Circular dichroism comparing the secondary...
<p>A) As purified rNbp-35 and rNbp35 after chemical reconstitution was scanned in the UV-Vis range t...
<p>(A) SDS-PAGE analysis of representative fractions after solubilization of the membrane preparatio...
Spectra were recorded at room temperature of GPRs reconstituted into E. coli polar lipids membrane v...
<p>Proteins were extracted from purified MLBs, solubilized with denaturation solutions, and run on a...
In high-throughput screening (HTS) assays, the use of ultraviolet absorption spectroscopy (UA) is co...
<p>A. Absorption spectra of the non-mutated proteins. B. Absorption spectra of the mutants. C, D. Ab...
Purified wild-type (WT) recombinant Nus-BB0562, single (S34A; S34T; S58A; S58T) and double mutant (S...
<p>Spectra of RAL 20 µM (black) together with 20 µM LTR32 (green), 20 µM LTR34 (blue), 20 µM LTR30 (...
<p>Rtms5<sup>Y67F</sup> (A) and Rtms5<sup>Y67F/H146S</sup> (B) were diluted to a final protein conce...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
<p>Absorption spectra of purified proteins were taken between 260–600 nm wavelengths. A; VcCry1-MBP....
<p>Spectra were normalized to the 280 nm absorption for each protein. <b>A.</b> Absorption spectra o...
<p>A) Protein purity assessed on SDS-PAGE. MW: molecular weight standard from Euromedex; 1: Cfp21 WT...
<p>(A, B) Far-UV CD spectra and fluorescence spectra of folded and unfolded NS1 (full and dashed lin...
<p>(A) SDS-PAGE of each purified recombinant protein. (B) Circular dichroism comparing the secondary...
<p>A) As purified rNbp-35 and rNbp35 after chemical reconstitution was scanned in the UV-Vis range t...
<p>(A) SDS-PAGE analysis of representative fractions after solubilization of the membrane preparatio...
Spectra were recorded at room temperature of GPRs reconstituted into E. coli polar lipids membrane v...
<p>Proteins were extracted from purified MLBs, solubilized with denaturation solutions, and run on a...
In high-throughput screening (HTS) assays, the use of ultraviolet absorption spectroscopy (UA) is co...
<p>A. Absorption spectra of the non-mutated proteins. B. Absorption spectra of the mutants. C, D. Ab...
Purified wild-type (WT) recombinant Nus-BB0562, single (S34A; S34T; S58A; S58T) and double mutant (S...
<p>Spectra of RAL 20 µM (black) together with 20 µM LTR32 (green), 20 µM LTR34 (blue), 20 µM LTR30 (...
<p>Rtms5<sup>Y67F</sup> (A) and Rtms5<sup>Y67F/H146S</sup> (B) were diluted to a final protein conce...
SDS-PAGE analysis of the purified recombinant proteins used in this study. M = molecular mass marker...
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