Identification of purified recombinant protein as mPRα.

<p>(A) SDS-PAGE analysis of representative fractions after solubilization of the membrane preparation (DDM solubilized), column chromatography over Ni-NTA, amino cellulose (Cellufine Amino). Protein bands were detected by CBBR staining. (B) SDS-PAGE analysis of purified hmPRα. Protein bands were detected by CBBR staining (CBBR) or were immunostained by anti-His-tag antibody (α-His). An arrow indicates hmPRα. (C) MALDI-TOF mass spectrum of purified hmPRα. (D) Amino acid sequence of recombinant hmPRα from produced in this study. The matching peptides from the peptide mass fingerprint analysis are underlined. The sequences of α-factor signal, c-Myc-tag and His-tag are boxed.