Absorption spectra of purified proteins.

<p>Absorption spectra of purified proteins were taken between 260–600 nm wavelengths. A; VcCry1-MBP. MTHF absorption at 380 nm and the minor peaks between 420–480 nm were the indication of different oxidation states of FAD in this purification. B; VcPhr-MBP. The absorption peaks at 380, 420, 440 and 480 nm shows reduced, oxidized, neutral radical forms of FAD existence in the purified VcPhr.