The dose responses for the whole-body edematous effects of carrageenan in adult zebrafish.

<p>Time course and the percentage change of whole-body volume induced by i.p. injection of vehicle (PBS) or carrageenan in adult zebrafish (A), using a rat paw volume meter. The basal whole-body volume of each adult zebrafish before i.p. injection of vehicle or carrageenan injection was considered as 100%, and the increase in whole-body volume is presented as a percentage change from the basal values by subtracting the basal whole-body volume from the whole-body volume measured at each time point. The absolute basal whole-body volume of adult zebrafish before the injection of vehicle or carrageenan is 593.9±49.3 µL (<i>n</i> = 36). The area under the abdominal-edematous-effect time curve (B), which was calculated from Figure A, for i.p. vehicle or carrageenan (0.75%, 1.5%, and 3%) injection extended from 0 to 8 h after i.p. carrageenan injection and showed that i.p. 0.75–3% carrageenan induced a significant increase in the whole-body volume in adult zebrafish. Each point or bar in all figures represents the mean ± SEM of 9 adult zebrafish per group. AUC: area under the curve; 0.75% Ca: 0.75% carrageenan; 1.5% Ca: 1.5% carrageenan; 3% Ca: 3% carrageenan; <b>*</b><i>P</i><0.05 compared with the same time points after i.p. injection of the vehicle (PBS) group; +<i>P</i><0.05 compared with the same time points after i.p. injection of the carrageenan (0.75%) group; #<i>P</i><0.05 compared with the same time points after i.p. injection of the carrageenan (1.5%) group.