Number of crossings per minute of adult zebrafish through the longitudinal line of the tank after being treated with different anaesthetic protocols.

<p>The activity of zebrafish was analysed at A) 5 h and B) 24 h after being subjected to the following anaesthetic baths: 100 μg/mL MS-222 (MS), 0.2 μg/mL etomidate (E); 0.2 μg/mL etomidate + 100 μg/mL lidocaine (E+L); 1.25 μg/mL propofol (P); 1.25 μg/mL propofol + 100 μg/mL lidocaine (P+L); 100 μg/mL ketamine (K); 100 μg/mL ketamine + 1.25 μg/mL medetomidine (K+M); or 100 μg/mL ketamine + 1.25 μg/mL medetomidine / 3.125 μg/mL atipamezole (K+M/A). A group of non-anaesthetized animals served as Control. Each point represents an animal (n = 4 for control group; n = 8 for all other groups). Data are expressed as median [interquartile range]. *p<0.05 when compared with Control. Non-parametric Kruskal-Wallis test followed by Dunn's multiple comparisons test.