Chemical unfolding was monitored by CD and fluorescence.

<p>Fraction unfolded at 298 K temperature computed by a two-state model using <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103579#pone.0103579.e004" target="_blank">equations (4)</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103579#pone.0103579.e006" target="_blank">(6)</a> using CD (○) (specific ellipticity at 227 nm, 5 µM lectin) and Fluorescence (□) (emission intensity at 360 nm, 5 µM protein) for CLA as a function of the denaturant concentration at pH 2 (A) and pH 7 (B). (Insets) Concentration dependent GdnCl-induced unfolding monitored by change in fluorescence emission at 360 nm at pH 2 and pH 7 with 2 µM (○) and 10 µM (□) lectin respectively.